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Single step fractionation of raw milk with FraMiTrACR® prior to detection of the residual contaminants chlorate and perchlorate

Authors

Jan-Michael Steils pureMilk analytical GmbH https://orcid.org/0000-0001-8782-6275
Christian Baumgartner pureMilk analytical GmbH https://orcid.org/0000-0002-3725-8414
Klaus Schöne Sartorius Lab Instruments GmbH & Co.KG https://orcid.org/0000-0002-9160-9211
Maren Lang Milchprüfring Baden-Württemberg e.V.
Melina Kraus Milchprüfring Baden-Württemberg e.V.
Harald Thenmaier Sartorius Austria GmbH
John Cashman Sartorius UK Ltd., https://orcid.org/0000-0002-7781-8690

Keywords:

Milk, Raw Milk, Chlorate, Perchlorate, Sample Preparation, Fractionation, LC-MS/MS

Abstract

Residues of chlorate and perchlorate in dairy products are causing challenges for stakeholders in the dairy food chain. Both residues enter the dairy food chain almost exclusively as a disinfection or cleaning by-product. As these substances can endanger food safety, a cost-effective, passive and rapid raw milk sample preparation workflow was developed. By means of centrifugal ultrafiltration, unprocessed raw milk was fractionated into its constituent phases (water and fat/protein), using a FraMiTrACR®° unit. When the water phase prepared by this method was analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS), it was possible to achieve quantification and detection levels of 0.001 mg/kg and 0.0005 mg/kg, respectively, for perchlorate, and quantification and detection levels of 0.01 mg/kg and 0.005 mg/kg, respectively, for chlorate.

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